Stool Culture

Stool Culture, This is a preparation in which bacteria grow. There are nutrients for bacteria. A medium in which bacteria have been added. It is essential for the growth and recognition of bacteria. Different bacteria form colonies of different shapes and colors. 

Purpose of Stool Culture:

The primary purpose of a stool culture is to diagnose infections in the digestive tract that may be causing symptoms such as diarrhea, abdominal pain, nausea, or vomiting. By identifying the specific microorganism responsible for the infection, healthcare providers can determine the most appropriate treatment, such as antibiotics for bacterial infections or antiparasitic medications for parasitic infections. Stool cultures can also help detect conditions like food poisoning, gastrointestinal infections, and other digestive disorders.

Procedure:

Day-0:

A small amount of stool is taken, placed in a test tube, diluted with Peptone water, and then cultured on mechanical agar DCA tetrathionate broth. In addition, if this sample is from a child under 5 years of age, it is also applied to blood agar.

If cholera is suspected, it is put on TCBS. TCBS refers to Thiosulphate Citrate Bile Salt Sucrose Medium, All media are incubated at 37°C for 24 hours in the presence of oxygen.

Day-1:

A culture stomach is examined to identify if any growth has occurred. For this, a smear slide is made and sensitized.

Day-2:

After identifying it, a report is prepared by applying more sensitivity.