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Sterilization and Disinfection

Sterilization and Disinfection, Sterilization is defined as the complete pilling or removal of all visible microorganisms and Spores from an object or a particular environment.

Disinfection: Disinfection is defined as the killing, inhibition, or removal of pathogenic microorganisms (usually in inanimate or non-living objects).

Difference:

Terms of Comparision Sterilization Disinfection
Definition The Process of Complete killing or removal of all living microorganisms The Process of Complete killing or removal of all living microorganisms
Mode of Action They kills both vegetative cells and spores. They kill both vegetative cells and spores.
Type of Methods They kill both vegetative cells and spores Chemical methods are used for disinfection
Examples Heat (dry and moist)
Radiation
Filtration
Chemical
Alcohol
Chlorine Compounds
Formaldehyde
FoHydrogen Peroxide
Chemical agents used to carry out sterilization are called sterilants Chemical agents used to carry out sterilization are called sterilants Chemical agents used to carry out sterilization are called sterilants
Methods Chemical agents used to carry out sterilization are called sterilants
Physical Method: Chemical Method:
Thermal Gaseous
Filtration Liquid
Radiation
There are two methods of disinfection
1. Thermal Process
2. chemical Process
Applications Used mainly for decontamination in food Medicine and Research kill my deactivate all forms of life microorganisms Like bacteria
Fungi
Spores
Fluids
Used in daily life like decontamination
Surface and air
To destroy or inhibit the growth of microorganisms on the surface of an instrument or surgical device
Used in the water industry

Food processing
Use in hospitals
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5 thoughts on “Sterilization and Disinfection”
  1. […] Two drops of fresh blood on a glass slide. After that capillary tube aged with blood. Blood will automatically fill the capillary tube. Leave the tube slightly empty. And broke a small part of the tube at ten-second intervals. And see if there is a clotting blood or not. Note the time when the blood clot is visible. This will be your endpoint and clotting time. […]

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