MCQs about Culture Media
MCQs about Culture Media, These are fifty multiple-choice questions (MCQs) about culture and media, with the right answer for each given. These questions are essential for medical laboratory students.
- Which culture media is used to grow most types of bacteria and fungi?
- A) MacConkey agar
- B) Blood agar
- C) Sabouraud agar
- D) Nutrient agar
- Answer: D) Nutrient agar
- Which media is selective for Gram-negative bacteria?
- A) Mannitol salt agar
- B) MacConkey agar
- C) Blood agar
- D) Chocolate agar
- Answer: B) MacConkey agar
- Which media differentiates bacteria based on their ability to ferment lactose?
- A) MacConkey agar
- B) EMB agar
- C) Blood agar
- D) Sabouraud agar
- Answer: A) MacConkey agar
- Which agar is used to identify and differentiate Staphylococcus aureus?
- A) Mannitol salt agar
- B) Blood agar
- C) Sabouraud agar
- D) Chocolate agar
- Answer: A) Mannitol salt agar
- Which media is used to culture fungi?
- A) Blood agar
- B) Sabouraud agar
- C) MacConkey agar
- D) EMB agar
- Answer: B) Sabouraud agar
- Which media is used for growing fastidious organisms such as Neisseria and Haemophilus?
- A) Nutrient agar
- B) MacConkey agar
- C) Blood agar
- D) Chocolate agar
- Answer: D) Chocolate agar
- What type of media is Hektoen enteric agar?
- A) Selective and differential
- B) Enriched
- C) General purpose
- D) Transport
- Answer: A) Selective and differential
- Which media is used for the detection of coliforms and enteric pathogens?
- A) Blood agar
- B) EMB agar
- C) Nutrient agar
- D) Sabouraud agar
- Answer: B) EMB agar
- Which media can differentiate between lactose fermenters and non-fermenters based on the color change?
- A) MacConkey agar
- B) Nutrient agar
- C) Blood agar
- D) Chocolate agar
- Answer: A) MacConkey agar
- What is the primary use of blood agar?
- A) To grow fungi
- B) To culture fastidious bacteria
- C) To differentiate lactose fermenters
- D) To isolate Gram-negative bacteria
- Answer: B) To culture fastidious bacteria
- Which medium is used to differentiate bacteria based on hemolysis?
- A) Mannitol salt agar
- B) Blood agar
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) Blood agar
- What type of medium is Sabouraud agar?
- A) Selective
- B) Differential
- C) Enriched
- D) General purpose
- Answer: A) Selective
- Which medium is specifically designed to grow dermatophytes?
- A) MacConkey agar
- B) Blood agar
- C) Sabouraud agar
- D) Chocolate agar
- Answer: C) Sabouraud agar
- What is the purpose of using mannitol salt agar?
- A) To grow fungi
- B) To differentiate hemolytic bacteria
- C) To isolate Staphylococcus species
- D) To differentiate lactose fermenters
- Answer: C) To isolate Staphylococcus species
- Which medium contains bile salts and crystal violet to inhibit Gram-positive organisms?
- A) MacConkey agar
- B) Blood agar
- C) Chocolate agar
- D) Sabouraud agar
- Answer: A) MacConkey agar
- Which media is used to isolate Mycobacterium species?
- A) Nutrient agar
- B) Lowenstein-Jensen agar
- C) MacConkey agar
- D) Blood agar
- Answer: B) Lowenstein-Jensen agar
- Which medium is used to differentiate organisms that produce hydrogen sulfide?
- A) MacConkey agar
- B) Blood agar
- C) Triple sugar iron agar
- D) Chocolate agar
- Answer: C) Triple sugar iron agar
- Which medium is enriched with vitamins, amino acids, and other growth factors for fastidious bacteria?
- A) Nutrient agar
- B) Blood agar
- C) Chocolate agar
- D) MacConkey agar
- Answer: C) Chocolate agar
- Which medium is selective for fungi and inhibits bacterial growth?
- A) Nutrient agar
- B) Blood agar
- C) MacConkey agar
- D) Sabouraud agar
- Answer: D) Sabouraud agar
- Which media contains eosin and methylene blue dyes?
- A) Blood agar
- B) EMB agar
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) EMB agar
- Which medium differentiates enteric bacteria based on hydrogen sulfide production and carbohydrate fermentation?
- A) MacConkey agar
- B) Triple sugar iron agar
- C) Blood agar
- D) Sabouraud agar
- Answer: B) Triple sugar iron agar
- Which media is used for the selective isolation of Gram-positive organisms?
- A) MacConkey agar
- B) PEA (Phenylethyl Alcohol) agar
- C) EMB agar
- D) Chocolate agar
- Answer: B) PEA (Phenylethyl Alcohol) agar
- Which medium is used to test for the presence of urease enzyme in bacteria?
- A) MacConkey agar
- B) Blood agar
- C) Urea agar
- D) Chocolate agar
- Answer: C) Urea agar
- Which medium differentiates bacteria based on their ability to decarboxylate amino acids?
- A) Blood agar
- B) MacConkey agar
- C) EMB agar
- D) Lysine iron agar
- Answer: D) Lysine iron agar
- Which media is used for the isolation of Salmonella and Shigella species?
- A) Blood agar
- B) Hektoen enteric agar
- C) Nutrient agar
- D) Chocolate agar
- Answer: B) Hektoen enteric agar
- Which media contains bile salts and dyes to differentiate lactose fermenters from non-fermenters?
- A) MacConkey agar
- B) Blood agar
- C) Sabouraud agar
- D) Chocolate agar
- Answer: A) MacConkey agar
- Which media is used for the detection of Gram-negative enteric bacteria?
- A) Blood agar
- B) MacConkey agar
- C) Nutrient agar
- D) Sabouraud agar
- Answer: B) MacConkey agar
- Which medium is used to detect the presence of indole in bacteria?
- A) Blood agar
- B) MacConkey agar
- C) SIM (Sulfide Indole Motility) agar
- D) Chocolate agar
- Answer: C) SIM (Sulfide Indole Motility) agar
- Which medium contains mannitol and phenol red as pH indicators?
- A) Blood agar
- B) Mannitol salt agar
- C) MacConkey agar
- D) EMB agar
- Answer: B) Mannitol salt agar
- Which medium is used to detect citrate utilization by bacteria?
- A) MacConkey agar
- B) Simmon’s citrate agar
- C) Blood agar
- D) Chocolate agar
- Answer: B) Simmon’s citrate agar
- Which medium differentiates bacteria based on their ability to hydrolyze starch?
- A) Blood agar
- B) MacConkey agar
- C) Starch agar
- D) Chocolate agar
- Answer: C) Starch agar
- Which media is used to culture anaerobic bacteria?
- A) Blood agar
- B) MacConkey agar
- C) Thioglycollate broth
- D) Chocolate agar
- Answer: C) Thioglycollate broth
- Which medium is used for the cultivation of Corynebacterium diphtheriae?
- A) Blood agar
- B) Loeffler’s serum slope
- C) MacConkey agar
- D) Sabouraud agar
- Answer: B) Loeffler’s serum slope
- Which medium is used to detect gelatin hydrolysis by bacteria?
- A) Blood agar
- B) MacConkey agar
- C) Gelatin agar
- D) Chocolate agar
- Answer: C) Gelatin agar
- Which medium is used to differentiate Vibrio species based on sucrose fermentation?
- A) Blood agar
- B) MacConkey agar
- C) Thiosulfate-citrate-bile salts-sucrose (TCBS) agar
- D) Chocolate agar
- Answer: C) Thiosulfate-citrate-bile salts-sucrose (TCBS) agar
- Which medium is selective for the isolation of pathogenic Neisseria species?
- A) Blood agar
- B) MacConkey agar
- C) Thayer-Martin agar
- D) Chocolate agar
- Answer: C) Thayer-Martin agar
- Which medium is used to detect bacterial motility?
- A) Blood agar
- B) SIM (Sulfide Indole Motility) agar
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) SIM (Sulfide Indole Motility) agar
- Which medium is used to grow Bordetella pertussis?
- A) Blood agar
- B) Regan-Lowe agar
- C) MacConkey agar
- D) Sabouraud agar
- Answer: B) Regan-Lowe agar
- Which medium is used to isolate and differentiate Yersinia species?
- A) Blood agar
- B) CIN (Cefsulodin-Irgasan-Novobiocin) agar
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) CIN (Cefsulodin-Irgasan-Novobiocin) agar
- Which medium differentiates bacteria based on their ability to produce acid from glucose fermentation?
- A) Blood agar
- B) MacConkey agar
- C) Methyl red-Voges Proskauer (MR-VP) broth
- D) Chocolate agar
- Answer: C) Methyl red-Voges Proskauer (MR-VP) broth
- Which medium is used to isolate Legionella species?
- A) Blood agar
- B) Buffered charcoal yeast extract (BCYE) agar
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) Buffered charcoal yeast extract (BCYE) agar
- Which medium contains sodium thiosulfate and ferric citrate for hydrogen sulfide detection?
- A) MacConkey agar
- B) XLD (Xylose Lysine Deoxycholate) agar
- C) Blood agar
- D) Chocolate agar
- Answer: B) XLD (Xylose Lysine Deoxycholate) agar
- Which medium is used to culture Campylobacter species?
- A) Blood agar
- B) skidrow’s agar
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) skidrow’s agar
- Which medium is used for the isolation of Mycoplasma species?
- A) Blood agar
- B) MacConkey agar
- C) PPLO (Pleuropneumonia-Like Organisms) agar
- D) Chocolate agar
- Answer: C) PPLO (Pleuropneumonia-Like Organisms) agar
- Which medium is used to differentiate bacteria based on their ability to utilize malonate?
- A) Blood agar
- B) MacConkey agar
- C) Malonate broth
- D) Chocolate agar
- Answer: C) Malonate broth
- Which medium is used to test for the presence of oxidase enzyme in bacteria?
- A) Blood agar
- B) Oxidase test agar
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) Oxidase test agar
- Which medium is used for the detection of DNase activity in bacteria?
- A) Blood agar
- B) DNase agar
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) DNase agar
- Which medium is used to differentiate bacteria based on the production of hydrogen sulfide, indole, and motility?
- A) Blood agar
- B) SIM (Sulfide Indole Motility) agar
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) SIM (Sulfide Indole Motility) agar
- Which medium is used to isolate Brucella species?
- A) Blood agar
- B) Brucella agar
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) Brucella agar
- Which medium is used to culture anaerobic bacteria with a reducing agent?
- A) Blood agar
- B) Robertson’s cooked meat medium
- C) MacConkey agar
- D) Chocolate agar
- Answer: B) Robertson’s cooked meat medium