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Ketone Bodies are produced by the liver and used peripherally as an energy source when glucose is not readily available. The two main ketones are acetoacetate (AcAc) and 3-beta-hydroxybutyrate (3HB), while acetone is the third, and least abundant, ketone body.

ketone bodies test is the severe Uncontrolled Diabetes mellitus starvation:

Rothera’s test, Gerhardt’s test: Acetone, Acetoacetate, Acetoacetate:

Tags:

Rothera’s Test:

Procedure:

  • Take 5ml of Urine, and Satucate it with ammonium sulfate.
  • Add a drop of freshly prepared Sodium nitroprusside and Mix.
  • Add 3 ml of strong ammonia carefully by the side of the tube.

Observation:

Interference:

  • Acetone Acetoacetate Present:

Gerhardt Test:

Procedure:

  • Take 3ml of Urine Add ferric chloride dropwise

Observation:

  • No Portwine color (red color)

Interference:

  • Acetoacetate is absent
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