Heller’s Test

Heller’s Test

Heller’s Test is a biochemical test performed to detect proteins in a sample by the denaturation of these proteins by the addition of strong acids. It is a qualitative test for the detection of protein in the urine.

Principle of Heller’s Test:

The test is based on the principle of precipitation of proteins, Concentrated Nitric Acid Denature protein, and forming a white precipitate when urine comes in contact with the Nitric Acid.

Symptoms Of Heller’s Test:

Affected children show clinically significant the following terms:

• Child Hood disintegrative disorder (CDP).
• Social function.
• Development delays in language.

Requirements for Heller’s Test:

• Concentrated Nitric Acid,
• Dropper, Test Tubes,
• Test tube stand,
• Urine (5ml),
• Pipettes,

Procedure:

Take 2ml of concentrated nitric and in a test tube Add urine drop by drop by the side of the test tube. The appearance of a white ring at the junction indicates the presence of protein Because nitric acid is a denatured protein and a white ring is formed at the junction. So this means the Test is positive for protein in the urine.

Result:

Positive Result:

The positive test is represented by the formation of a white ring at the junction of the two distinct layers, It indicates the presence of proteins in the given sample.

Negative Result:

A negative result is represented by the absence of a white ring. This indicates the absence of protein in the sample.

Limitations:

The concentrated and used in the test is Corrosive and thus, should be handled carefully The test is qualitative and doesn’t give the concentration of protein present in the sample.