Creatinine Test in Blood
Creatinine Test in Blood, The serum creatinine test measures the amount of creatinine in the blood. Serum Creatinin is a waste product, Produced in the muscles, after creatinin breakdown. Creatinine is organic and helps to supply energy to muscle cells. It is synthesized mainly in the liver from arginine, glycine, and methionine. Once formed creatinine is not reused in the body’s metabolism and thus functions solely as a waste Product. The more muscle a person has more creatinine they Produce. A creatinine test measures how well your kidneys on Performing their job of filtering waste from your body’s blood.
Principle of Creatinin Test:
The main Principle of the creatinine test is it can be determined in serum or urine.
Jaffe’s Reaction:
Jaff’s reaction where the creatinine Produces an orange color with Picric acid in an alkaline medium.
Other Names of Creatinin Test in Blood:
Also, known Also called Serum Creatinine, Plasma Canalinin, and Blood Creatinine.
Reference Range of Creatinin in Blood:
| Between Infants female & males | Value / Range | Unit | |
| a | Less than 2 years | 0.1 – 0.4 | mg/dL |
| b | 2 years to < 6 years | 0.2 – 0.5 | mg/dL |
| c | 6 years to 10 years | 0.3 – 0.6 | mg/dL |
| d | 10 years to < 18 years | 0.4 – 1.0 | mg/dL |
| e | Females 18 years or older | 0.5 – 1.0 | mg/dL |
| f | Males 18 years or older | 0.6 – 1.2 | mg/dL |
Causes of High Creatinine, Seen in Blood:
- Nephrons Damage
- Acromegaly
- Gigantism
- Hyperthyroidism
- Congestive Heart failure
- Urinary tract obstruction
- Chronic and Acute Renal failure
- Poliomyelitis Burns
Low level of Creatinin, Causes in Blood:
- Severe Liver Disease
- Low Protein Diet
- During Pregnancy
- Increasing Age
- Muscular Dystrophy
Diagnostic Importance:
→ To screen a suspected disorder involving muscle
→ To diagnose kidney disease
→ To evaluate kidney function
→ Screen Glomerular filtration
→ Creatinine is a more reliable indicator of renal function
How to Perform the Test?
- By using a Blood sample (In a vial)
- By using a urine sample (In Urine Container)
- Chemical Tests Through Reagents or Buffers
- Microscopic Examination (Through microscope)
- Manual Performance
Sample Precautions:
Serum/Plasma: There is no need for any Preparation.
Manual Procedure:
| Reagents | Assay | Standard |
| R-1 | 500 μL | 500 μL |
| R-2 | 500 μL | 500 μL |
| Standard | — | 100 μL |
| Serum | 100 μL | — |
First reading after 30 seconds and reading after to second out 520nm.
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