Prothrombin Time PT means that the blood is taking too long The prothrombin time sometimes referred to as PT ON pro time test is a test to evaluate blood clotting. It is a protein produced by your liver. It is one of many factors in your blood that help it to clot appropriately.
The calcium in whole blood is removed by sodium citrate and prevents clotting but they add tissue thromboplastin and cacti in the plasma It will clot finally we note the clotting time. It is vitamin K dependent. It evaluates Extrinsic and Common pathways See how well warfarin is working warfarin is a blood thinner medicine that is used to treat and prevent dangerous blood clots.
It is used to check clotting functions before surgery when uniplastin reagent is added to normal plasma, clotting. The mechanism is initiated by forming a gel clot within a specific period.
Causes PT Levels to Rise?
- Liver disease
- Deficiency of Vitamin “k”
- Deficiency of Coagulation factor (eg factor vi deficiency)
- There are some clotting factors like (VII, X, II, IX)
PT Symptoms?
- Poor Posture
- Chronic Headaches
- Joint Pain
- Difficulty recovering from injuries
When would You Need the PT Test:
- Bleeding in your poop or urine,
- Gums bleeding,
- Nose bleeding easily,
- Heavy menstrual period in women,
- Bleeding or bruising easily,
How to Performed this Test:
Requirement:
- Platelet Poor Plasma
- Thromboplastin
- Ca cl2
- Stopwatch
- Glass vial
- Water bath for 37°C
Procedure:
Take 100 μL of plasma in a glass test tube. Place in a water bath or incubator for 5 minutes. After 5 min Add 200 μl of PT reagent (Tissue thromboplastin) Pre-warmed at 37°C for at least 3 minutes. Start the stopwatch and shake the tube gently to mix the content. By tilting the tube note clotting time.
Normal Range:
Normal range of 11 to 16 Seconds PT, INR (International normalized ratio). An INR is A type of calculation based on PT test results.
INR: Patient PT, Control PT,
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